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| Alignment: Delivery Options |
Step Five in Aligning Your Sequences:
1. Enter a valid email address in the space
provided. This is the address to which your NAST aligned FASTA file
and Excel report will be sent.
2. By default, the files returned to you by Greengenes will be compressed
before they are sent. As the files are rather large please leave
this default selected as is. However, if you know that your mail server
blocks zip compressed files you will need to either change that setting
if you can or unclick this option.
3. Once you have entered your email
address you can click on "Process Batch". In the absence of
high traffic you can expect your NAST aligned FASTA & Excel
files to be sent within 10
minutes. (When you receive your NAST summary your computer will
want to open it with Excel. That's fine; go ahead and open it
with Excel and look at it. However, it is not saved in the
default Excel workbook format; it is "tab delimited". This won't
matter to you except that if it asks you if you want to save changes
when you close it, choose 'NO'. When you proceed to the next
step, eliminating chimeric sequences, you need to have a "tab
delimited" NAST summary. This is what is returned to you from
Greengenes. You will be asked to submit it along with your NAST
aligned FASTA file to expidite the chimeric check.)
4. To see a sample Greengenes Excel spreadsheet with a complete description of the columns click here.
5. Once you have your aligned FASTA file
you can continue with checking for and eliminating chimeric sequences.
Click here to proceed with directions on
how to check for chimeras.
Alignment
Tutorial Main
Greengenes Main
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