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Methods
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How greengenes is maintained:
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One purpose of the greengenes project is to computationally align all publicly available 16S sequences and place them
phylogenetically. A scriptable, scalable, alignment method was conceived, NAST, producing a
global
16S rRNA gene Multiple Sequence Alignment. Over 9,000 Operational Taxonomic Units (OTUs) have been identified solely by
sequence identity clustering. Details of the algorithms that were used were described in the journal Bioinformatics.
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